Do you have a plan, or are you just going to add the stuff and see what happens?
I feel that if i tried this, I would set up several carboys with slightly different environments to hedge against one of them failing. Though, in reality I would probably just end up doing a single one.
I'm not entirely sure yet. I have 50 seeds of 4 different species coming and am thinking about approaching this in one of two ways.
1) I have dozens of pots in my garage and was planning on putting a dozen or seeds from each species in each pot. In some pots I will mix the species. After several weeks I will see which plants are doing the best and assume that those are the heartiest/most likely to survive. They will all be growing under controlled conditions in my basement. I can't let them get too big though, or else they'll be difficult to get in the carboy (This may prove to be problematic regardless what size they are).
2) Germinate as many seeds as I can, start filling the carboy with soil, randomly dropping seeds at different depths, and hope for the best. Whichever seed is the strongest and most likely to survive will prevail.
I might go with method number 2, but grow some potted ones anyway. That way, when I'm ready to plug the carboy up, I'll have a bunch of plants I could kill and drop in prior. That will be in hopes of giving bacteria something to initially feed on and hopefully start generating some C02 in the sealed bottle. Whichever method I end up doing, I probably wont seal the carboy until the plant is fairly established and healthy.
I would really love it if I could get some sort of insect or organism (any kind would be awesome) that could live off the environment as well. It'd need to be able to reproduce fairly easily. Ants would be cool, but they require a queen for reproducing. I think it would be fantastic if, hypothetically speaking, 50 years from now I had a self sustaining ecosystem with plants and organisms that are of true genetic lines.
Any input would be very cool.