Even before the movie, Queen was responsible for a whole lot of universally popular songs.
Bohemian Rhapsody
We Will Rock You
We Are the Champions
Fat Bottom Girls
Crazy Little Thing Called Love
etc
Even if you didn't know some of them were queen, a ton of people are familiar with those songs. So it's really not fair to credit the movie for more than just a small bump in younger people.
Well, "popular" and "second most popular band ever" are not the same. There's no doubt they were popular, but just because Rock And Roll, Part I is played at every sports event ever in the States doesn't mean that Gary Glitter is at the level of the Beatles.
Queen has FIVE iconic songs: BoRhap, WWRY/WATC; Crazy..., and Another One Bites The Dust. The artists that have that are legion.
Zeppelin: Stairway; WLL; Rock and Roll; Black Dog; Kashmir (and you can sub in any of about 5 other songs too)
Floyd: Comfortably Numb, Money, Wish You Were Here, Hey You, Time (or ...Brick)
Skynyrd: Freebird, Sweet Home, Saturday Night Special, What's Your Name, Tuesday's Gone, Simple Man...
The Who: All three CSI tunes (Who Are You, .... Fooled, Baba O'Reilly) plus Pinball Wizard, My Generation...
John: Saturday Night's..., Candle In The Wind, Goodbye Yellow Brick Road, Daniel, Yuor Song (I can go foreever here)
Stones: Satisfaction, Jumpin' Jack, Brown Sugar, Start Me Up, Sympathy, Ruby Tuesday (I can go forever here too)
Queen is AWESOME. Fred is my favorite singer of all time (and in my view the greatest pure rock singer of all time). I have every CD - even Flash... even HOT SPACE! - and can find something redeeming about all of them. Queen II is a top ten record all time for me. So a lot of love for Queen, but this is a band that couldn't tour here for the last five (plus, minus) years of their career, and not just because of Freddie's illness. They are not at the level of Zeppelin or The Who in the general zeitgeist of America.